The Cultivation of Human Sarcomatous Tissue in Vitro

Isolation and in vitro cultivation of human skin Keratinocytes and preparation of epidermal sheet

O-19: Proliferation of Small Population of Spermatogonial Stem Cells in Azoospermic Patients

Background: With treatment success in young boys with cancer, long-term effects of cancer treatment have found importance in pediatric oncology. Temporary or permanent infertility after treatment is an important subject in childhood and adult cancer patients which decrease quality of life. The one approach to overcome infertility in these cases is to cryopreserve small biopsy testicular tissue ...

I-16: Assessment of The Vitrified Ovarian Tissue in Long Term Culture

In vitro culture of human ovarian tissue the following cryopreservation is proposed for follicular development. There are no techniques that guarantee successful maturation of the follicles within the excised tissue. The viability of cultured human ovarian tissue improved by adding some growth factors to the culture media. The efficiency of vitrification as the cryopreservation method for human...

Effect of Liver and Pituitary Digests on the Proliferation of Sarcomatous Fibroblasts of the Rat

1. A media containing all the essential constituents for the cultivation in vitro of sarcomatous fibroblasts of the rat has been prepared by digesting calf liver and also the anterior lobe of the pituitary body with pepsin. 2. The nutritive action of the pituitary digests is not altered by thorough extraction with ether. 3. After a pure strain of sarcomatous fibroblasts had been cultivated for ...

Isolation and in vitro Characterization of Mesenchymal Stem Cells Derived from the Pulp Tissue of Human Third Molar Tooth

Background: It is still controversial that the stem cells isolated from human dental pulp meets the criteria for mesenchymal stem cells (MSCs). The aim of the present study was to examine whether or not they are MSCs, or are distinct stem cells population residing in tooth pulp. Methods: Adherent fibroblastic cells in the culture of pulp tissue from human third molars were propagated through se...